TMIC-65. DISSECTING THE EXPRESSION AND FUNCTION OF ANGIOPOIETIN-1 IN BRAIN TUMORS

Abstract Angiopoietin-1 (Angpt1) is a secreted protein that can promote angiogenesis and vascular stability in development certain disease states through activation of the Tie2 receptor. However, little known about its expression or function across brain tumor types. Surveying pediatric adult tumors we show Angpt1 upregulated glial tumors, including high-grade gliomas ependymomas, patterns correlate with cell-type patters recently described normal developing brain. To examine cell type location within have generated electroporation based glioma ependymoma mouse models Angpt1-GFP reporter knock-in mice. found mainly cells both models, varying on driver mutations molecular subtype. Single-cell transcriptomic studies these are underway to further delineate non-tumor Moreover, leveraging this flexible electroporation-based modeling platform tumors. Preliminary data shows DNA plasmid encoding Cre-recombinase induces recombination loss our ongoing aim pathogenesis, blood-brain barrier function.